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Real Science Teaching. Real Classroom Experience.

I’m Amy Brown, a veteran high school biology and chemistry teacher, wife, and mom who understands the daily reality of lesson planning, grading, meetings, and everything in between. I know what it feels like to have too much to do and not enough time to do it.

After decades in the classroom, I’ve created rigorous, classroom-tested biology and chemistry resources that save you planning time while still delivering strong, meaningful science instruction. Every lab, activity, and lesson is designed to move students beyond memorization and into real scientific thinking.

If you want your students excited about science and thinking deeply without spending your entire weekend planning, you’re in the right place.

Amy Brown Biology and Chemistry Teacher

“I just love getting kids hooked on science.”

For My Mom, the Biology Teacher





My mom passed away in 2001, but not a day goes by that I don't think of her.  My mom was a biology teacher, and now I am a biology teacher, too.  My mom was an inspiration to so many people.  We grew up in a very small, rural town in the deep south.  Many of her students never dreamed they could attend college and have a different sort of life than the one they were accustomed to.  She taught them that they could do whatever ever they wanted if they worked hard enough.   She also taught them biology.
Growing up with my mother was quite the adventure!  At a very young age, I was looking at pond water under a microscope and marveling at the creatures I saw there.

I could spot liverworts and bladderworts growing so close to the ground in a swampy area that no one else would even know they existed there.  She always had her wildflower books with her, and the car would come to a screeching  halt whenever she spotted a potentially new wildflower along the road.

The car also stopped for turtles.  They simply had to be moved to the side of the road and out of harms way.  She showed me touch-me-nots and explained how touching them caused the cells to lose turgor pressure and that was why the leaves folded up.  Once on a family vacation to the beach, a dead sting ray washed up on shore.  We dissected it!  She took us fishing and canoeing and walking through the woods.  I caught lightening bugs like all kids do, but I knew the how and why of their flashing.

I have two daughters.  I have taken them on many of these same adventures.  They can amaze their friends with all sorts of biological  facts.  They love going on nature walks with me.  I thank my mother again for the closeness I share with my daughters.  She passed a love of nature to me, and as I passed this on to my daughters, we formed a strong and unbreakable bond to one another.

What choice did I have but to become a biology teacher, too?  I have now taught biology for 28 years.  I sincerely hope that I have inspired some young mind along the way.  Good luck to all my AP students who are taking the AP Biology exam tomorrow.

Thanks, Mom.  I love you.



PS - She was also an avid bird watcher.  She loved hummingbirds best of all.  Mom, this hummingbird is for you.

Reinforce Protein Synthesis with this Fun Game


Click image to view in my TpT store.

Sometimes when I teach  DNA, RNA, and protein synthesis, I feel like the only person in the room that "get's it" is me!    Many of my standard Biology I kids struggle with these concepts.  The whole idea of transcription and translation just leaves them looking like a deer in the headlights!  So I use this little activity to help get the point across.  It is called:

Determining the Traits of a Mystery Organism Through Protein Synthesis


After teaching the basics of protein synthesis, have your students work through this.  Given the DNA sequence of 6 genes, the student will determine the mRNA sequence, followed by the tRNA sequence.  Students will then de-code the codons to see what amino acids are needed to build a particular protein.  I have found that my students understand the concept of protein synthesis much better after doing this activity.  And best of all, they get to color their mystery organism at the end.  It never ceases to amaze me how much high school students still love to color!  This activity is appropriate for grades 7 through 10.


The printable lesson is perfect for traditional classroom settings, and the paperless, digital Google Apps version is perfect for distance learning and 1:1 classrooms. 

Transpiration Lab for High School Biology: Simple Plant Transpiration Experiment


Plant transpiration can be a challenging concept for high school biology students, especially when it is only taught through notes or diagrams. This simple plant transpiration lab helps students understand what plant transpiration is by observing water loss in leaves through an easy, hands-on classroom experiment. Designed for high school biology courses, this transpiration lab makes plant water movement visible, measurable, and meaningful.

I often find that my students are woefully lacking in their knowledge of plants. When time runs short at the end of the school year, plant units are usually the first to be cut. Because I enjoy teaching plants, I make it a priority to leave time each year for a plant unit.

This version of a plant transpiration lab is incredibly easy to set up, clean up, and take down. It uses a simple materials list and includes all the components I want in a lab activity, including data collection, graphing, critical thinking, problem solving, data analysis, and drawing conclusions. Plus, it is fun, and students always enjoy doing it.

TL;DR and want to see the activity in my TpT store? Click here.

What is Plant Transpiration?

Plant transpiration is the loss of water vapor from a plant through the leaves. As the stomata open to allow carbon dioxide to enter the leaf for photosynthesis, water vapor escapes the leaf and enters the atmosphere.

For trees and other tall plants, moving water from the roots to the top of the plant is a challenge. The major force in water transport is provided by the evaporation of water from the leaves during transpiration. As water is lost, the movement of water out of the leaf pulls water upward through the xylem all the way down to the roots. This process is known as transpiration pull.

Traditional Plant Transpiration Lab Using a Potometer

Traditional potometer setup showing plant stem and tubing used to measure water loss during a transpiration lab.

Traditionally, biology teachers used potometers to measure water loss from leaves during transpiration. Does this image bring back memories of a failed lab? Potometers are notoriously difficult to set up because it is hard to create a tight seal around the plant stem and rubber tubing. Without a good seal, results are unreliable, and students often crush the delicate stem during setup.


Simple plant transpiration experiment setup with bedding plants in beakers sealed with plastic bags to measure leaf water loss

Transpiration Lab for AP Biology and High School Biology

This simple plant transpiration lab is easy to set up, clean up, and take down, making it ideal for high school biology classrooms. Go to your local nursery or Walmart and buy some small bedding plants. I like to use begonia plants. The begonia is a good choice because it has a thick and fleshy leaf. 


Place the plants in small (250 mL) beakers. Water them thoroughly. In this experiment, you want to measure the amount of water lost from the leaves, so you have to ensure that no evaporation occurs from the soil in the beaker. Place a plastic sandwich bag around the beaker and wrap it very tightly around the stem of the plant. A bit of tape may be needed to keep the sandwich bag wrapped tightly around the stem. Only the leaf should be sticking out of the plastic bag.

The only way water is getting out of this beaker is through the leaves of this plant!

Determine the amount of water lost from the leaves by massing the entire setup. Be sure to get an initial mass before the experiment begins. In the photo shown, the initial mass was 195.57 grams.

Testable Variables in a Plant Transpiration Experiment


Now for the testable variables. What factors will cause an increase or decrease in the rate of transpiration? This lab will test 3 different environmental factors.
  • One plant will be placed in continuous light for 24 hours. 
  • One plant will be placed in front of a blowing fan for 24 hours.
  • One plant will be placed inside a plastic bag that has been spritzed with water to create high humidity.
  • The fourth plant will serve as a control.
Simple plant transpiration experiment setup showing experimental factors being tested: strong light, wind, and humidity.

Every experiment needs a control. In this transpiration lab, the control plant is not exposed to any experimental environmental factors. While the control plant is still transpiring, it does so under normal conditions, allowing students to compare results accurately.

Allow the plants to sit for 24 hours. After this time period, each plant is massed again. Students are often amazed at how much water has been lost through the leaves. All final masses should be compared to the control to determine whether the environmental factor tested caused an increase or decrease in the rate of transpiration.

There are also some uncontrolled variables in this experiment. The plants are not identical, and each has a different total leaf surface area, which can affect transpiration rates. Even so, students collect clear data showing the effects of environmental conditions on plant transpiration.

Adapting the Transpiration Lab for Different Grade Levels


Elementary: At the elementary level, it may be enough to demonstrate that water is absorbed by roots and released through leaves. Students can make predictions, form hypotheses, and practice steps of the scientific method.

Middle Grades: Middle school students can calculate the rate of water loss per minute, test additional variables such as darkness or temperature, or compare transpiration rates among different plant species.

High School Biology and AP Biology: 
High school students can remove leaves to calculate surface area and determine water loss per unit area. Students may also research mechanisms of transpiration such as cohesion, adhesion, capillary action, and transpiration pull.

After the experiment, I take the plants home and place them in my flower beds.

For additional plant kingdom resources, consider short video segments from The Private Life of Plants by David Attenborough. Many clips are available on YouTube and are typically three to five minutes long. Students always enjoy them.

Frequently Asked Questions About Plant Transpiration Labs

What is plant transpiration?
Plant transpiration is the process by which water moves from a plant’s roots, through the stem, and out of the leaves as water vapor. This water loss helps regulate temperature and supports the movement of water and nutrients throughout the plant.

What is a simple plant transpiration experiment for high school biology?
A simple plant transpiration experiment uses a live plant placed in a sealed container so that water loss occurs only through the leaves. By measuring mass before and after the experiment, students can observe and quantify transpiration.

How does this transpiration lab work for AP Biology?
This transpiration lab works well for AP Biology by allowing students to analyze variables such as light, humidity, air movement, and leaf surface area. Students can collect quantitative data, graph results, and connect observations to concepts like transpiration pull and water potential.

Why is a control important in a transpiration lab?
A control plant provides a baseline for comparison. By comparing experimental plants to a control, students can determine whether specific environmental factors increase or decrease the rate of transpiration.










Reviewing for the AP Biology Exam


Click picture to download PowerPoint.



The key to success is REVIEWING!!

If you have taught AP (Advanced Placement) Biology, then you know that the amount of material that must be covered before the exam is daunting.  I have had great success with my AP exam scores.  I normally have between 60 to 80 students take the exam each year.  For the 2011 exam, I had 65 students sit for the exam, and we had an average score of 4.3.  Admittedly, this is one of the best years I have ever had.  It was a great group of students who were highly motivated.  Those kids absorbed everything I threw at them.  They then dug deeper and deeper on their own outside of the regular class time.

The group I have this year makes me very proud!  I will have 61 taking the 2012 exam.  They are every bit as motivated and talented as the group I had last year.  They have such potential to do well!!  I just have to convince them how important it is to study and review for the exam.

I am convinced that the key to success is how you review the material with your students.  I began to review with my students on February 1.  I am most fortunate to have a 70 minute class each day.  I give a prayer of thanks for this each and every day, and I realize that most AP teachers do not have this luxury.  Beginning on February 1, I use the first 10 to 15 minutes of class to review a topic that we have previously covered earlier in the school year.  The remaining class time is used to cover new material.  The day before the AP exam in May, I am still reviewing and still covering new material!!

We have to convince our students that they must review, review, review before the exam.  I have written a set of powerpoints that I use for review. These are not teaching powerpoints.  These are just slide after slide of question and answer.  The students are not going to remember every little detail, so I try to make sure that they are solid on the basic facts.

Here is one of my review PowerPoints that I hope you will download.  It covers the characteristics of carbon and the organic compounds.



AP Review: Carbon and Organic Compounds ---- It's FREE!!  I hope you find this useful for your classroom.

Happy Teaching and good luck on the AP exam!

Everything About Enzymes!! (and a free lab!)



Recently, I was required to attend a system-wide inservice meeting for all the science teachers in our district.  In attendance were science teachers of grades 7 through 12.  These events are fun and interesting, mostly because I have been teaching for 28 years, and it is nice to see science teachers from other schools that I have known for years, but rarely get to see.  After joining up with a group of old acquaintances, the inevitable question came up:  "What have you been doing in your biology classes?"

I responded with, "I have just finished teaching a unit on enzymes to our biology 1 students."  One of the other teachers immediately responded with, "I don't really teach that in my class."

What????  How do you NOT teach about enzymes in a biology class????  As soon as I returned home that day, I promptly sent her all of my teaching materials on enzymes.

Enzymes are the stuff of life.  No cell would be alive without the action of enzymes.  Life in a cell is made possible through the hundreds of chemical reactions that occur there.  If these chemical reactions proceed too slowly, the activities of the cell would come to a screeching halt.  You see, enzymes are biological catalysts. They speed up the chemical reactions of the cell.  Without these enzymes, the reactions of the cell would proceed so slowly that they would be of no use to the cell, and the cell would die.

When is the best time to teach about enzymes?  I begin teaching about enzymes when I teach biochemistry.  When teaching about carbohydrates, lipids, and proteins, it is a natural fit to talk about enzymes as you discuss the structure and functioning of proteins.  I also teach about enzymes when I cover photosynthesis, respiration, replication, transcription, digestion..... This list could go on and on since enzymes are involved in every single biological process!

Be sure to cover all the basic points about enzymes:
  • Enzymes are biological catalysts that speed up the chemical reactions of the cell.
  • Enzymes are proteins.
  • Enzymatic reactions occur faster and at lower temperatures because enzymes lower the activation energy for that chemical reaction.
  • Enzymes are never consumed or used up during the reaction. They can do their job over and over again.
  • Enzymes are highly specific for just one substrate.  The enzyme has an active site with a unique 3-D shape into which this substrate must fit.  
  • Enzymes catalyze both the forward and the reverse of the same reaction.
  • Enzymes can be denatured by temperatures and pH levels outside the optimal range for that particular enzyme.
Enzymes are truly amazing proteins that play a vital role inside every living cell.  Please don't leave this out of your curriculum!

Okay.... I did promise you a freebie.  This is a lab that I have done for years, and it remains a favorite with my students year after year.

The Importance of Environmental Influences on Gene Expression



All students know that their hair color and their eye color is a result of the alleles that they have inherited from their parents. But do your students know that the environment plays a role in the expression of these alleles?

The phenotype of an individual often depends on the influences from the environment. A snowshoe rabbit has white fur in the winter months and brownish fur in the summer months. Why? If the rabbit is white in the winter, it will be camouflaged  and will have a greater chance of survival.  The same is true of a brown rabbit in the summer months.  A particular allele codes for coat color in these rabbits.  During the winter, the snowshoe rabbit will have white fur because these pigment producing genes do not function in cold weather.  When the weather warms in the spring and summer, the genes function to produce pigments and the coat becomes brown.

The million dollar question is this:  Is heredity or environment more important in determining the kinds of traits that appear in the offspring?

I recently had my students explore this very question in a laboratory activity. Students were given 10 corn seeds to plant.  Half of the students were asked to leave their container of planted seeds near a window in our lab.  The other half of the class was asked to place their container of corn seeds in complete darkness.   The seeds were watered as needed and left to grow.

The trait the students were asked to observe was the color of the stem and leaves.  Did the seeds grow into plants with green stems or into plants that were albino?  Stems are green due to the production of chlorophyll. Chlorophyll production is controlled by a dominant allele.  The absence of a dominant allele results in a plant that is albino.

The students were given seeds that were the offspring of heterozygous parents.  Therefore, it is expected that 3/4ths of the seeds will grow into corn plants that are green, and 1/4th of the seeds will grow into plants that are white or albino.

After allowing the seeds to grow into seedlings, students return to the lab to count their offspring.




In the above photos, both trays of corn were grown in the light.  As you can see, some of the plants have green stems, while other plants have albino stems.  Class data was tabulated and we were very close to the expected 3:1 ratio of green to albino stems.

Take a look at the plants in the photo to the right.  The plants on the right side of the photo were grown in complete darkness.  All of these plants are termed albino.  The expected 3:1 ratio was not supported.  100% of the plants grew into albino plants.  What is the difference between the albino plants grown in the light and the albinos grown in the dark?   The albino plants that were grown in the light are albino due to their genetics.  These plants have two recessive alleles for chlorophyll production.  Without the dominant allele, chlorophyll production is not possible.  The albino plants that were grown in complete darkness are albino because of their environment.  Some of these plants may have the dominant allele, but in the absence of sunlight, the dominant allele is not expressed.  

To make this argument just a bit stronger, all students were asked to let their seedlings continue to grow for another 24 hours, and all trays of seedlings were left in the light.  Plants that were initially grown in the dark, were left in the light for 24 hours.  At the second observation, 3/4ths of these albinos had developed chlorophyll and were green.

So back to the original question...... Is heredity or the environment more important in gene expression?  Every student in my class now knows that having the dominant allele is not always enough for the trait to be expressed. Sometimes the dominant allele and the proper environmental condition work together in the expression of the trait.

The printable lesson is perfect for traditional classroom settings, and the paperless, digital Google Apps version is perfect for distance learning and 1:1 classrooms. You might want to try this as a "Virtual Lab" for distance learning and 1:1 classrooms.  You can check out the "Virtual Lab Version" by clicking this link.

Give this lab a try.  Your students will love it!
Happy Teaching!


👉 Check this Blog Post: If your students need more guided practice with inheritance patterns, you may also want to try this free monohybrid cross worksheet with Punnett square practice problems.

👉 Check this Blog Post: Punnett Square Hidden Picture Activity
Students love reviewing genetics concepts with this engaging hidden picture reveal activity. As students solve Punnett square problems correctly, a hidden image is gradually revealed, making practice both interactive and motivating.