Amy Brown Science | Biology and Chemistry Teaching Resources for Middle and High School: photosynthesis
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Real Science Teaching. Real Classroom Experience.

I’m Amy Brown, a veteran high school biology and chemistry teacher, wife, and mom who understands the daily reality of lesson planning, grading, meetings, and everything in between. I know what it feels like to have too much to do and not enough time to do it.

After decades in the classroom, I’ve created rigorous, classroom-tested biology and chemistry resources that save you planning time while still delivering strong, meaningful science instruction. Every lab, activity, and lesson is designed to move students beyond memorization and into real scientific thinking.

If you want your students excited about science and thinking deeply without spending your entire weekend planning, you’re in the right place.

Amy Brown Biology and Chemistry Teacher

“I just love getting kids hooked on science.”

Showing posts with label photosynthesis. Show all posts
Showing posts with label photosynthesis. Show all posts

Cell Biology Warm Ups and Bell Ringers for High School Biology

The first few minutes of biology class can feel chaotic. Students are coming in, getting settled, finding materials, and waiting for class to begin. A well designed warm up can turn those first few minutes into meaningful science learning. Biology warm ups and bell ringers help students begin class with focused review while reinforcing important science concepts over time.

These cell biology warm ups and bell ringers were created for a high school biology unit covering cell structure, cell function, photosynthesis, cellular respiration, mitosis, and meiosis. They give students a consistent routine while also helping them review and reinforce important biology concepts throughout the unit.

Click here to see the Cell Biology Warm Ups and Bell Ringers in my TpT store.

Why I Use Warm Ups in Biology Class

Warm ups are one of my favorite classroom management tools because they help students begin class with a clear task. Instead of losing those first few minutes, students can review vocabulary, apply concepts, interpret diagrams, compare processes, or practice writing short answers.

These activities are especially helpful in a cell biology unit because there are so many connected ideas. Students need repeated practice with cell organelles, membrane transport, energy transfer, photosynthesis, respiration, mitosis, and meiosis. A short daily warm up gives students regular exposure to these concepts without taking over the entire class period.

Cell biology warm ups become a ready made final exam study guide for high school biology students

One of the best benefits is that the completed warm ups become a built in study guide. By the end of the unit, students have a collection of focused review pages that can be used before quizzes, tests, and semester exams.

What Is Included in These Cell Biology Warm Ups?

This cell biology warm up set includes 59 student pages designed for high school biology. The activities can be used as warm ups, bell ringers, exit slips, review pages, homework assignments, or short daily review activities.

The resource includes printable pages, editable student pages, digital Google Slides versions, teacher answer keys, and a teacher guide. The activities are designed to take about five to seven minutes and work best after students have received some instruction on the topic.

Cell biology warm ups covering cell structure photosynthesis respiration mitosis and meiosis

Topics Covered in These Biology Bell Ringers

Cell Structure and Function: Students review cell theory, plant and animal cells, organelles, surface area to volume ratio, cell membranes, membrane transport, and the organization of cells.

Photosynthesis: Students practice concepts related to energy flow, ATP, pigments, chloroplasts, light dependent reactions, the Calvin cycle, and plant adaptations.

Cellular Respiration: Students review glycolysis, the Krebs cycle, the electron transport chain, fermentation, ATP accounting, and the relationship between photosynthesis and respiration.

Cell Division: Students reinforce the cell cycle, mitosis, meiosis, chromosome number, asexual reproduction, sexual reproduction, and comparisons between mitosis and meiosis.

Sample warm up topics include cell membranes, mitochondria and chloroplasts, the Calvin cycle, glycolysis, ATP accounting, name that stage, and comparing mitosis to meiosis.

Printable and Digital Options

The printable version works well for students who keep a warm up notebook or binder. Each page is designed to be short enough for daily use while still giving students meaningful practice with important biology content.

Printable cell biology warm up notebook pages for high school biology

The digital version is included as Google Slides. This makes it easy to assign the warm ups through Google Classroom, use them in a paperless classroom, or display them for whole class review.

Editable digital Google Slides version of cell biology warm ups for high school biology

How These Warm Ups Help Students Review

Cell biology includes many abstract processes, and students often need repeated exposure before the ideas begin to connect. Warm ups give students a quick way to revisit earlier lessons while preparing for the next part of the unit.

I like using these activities because they give students practice with science vocabulary, diagrams, comparisons, short explanations, and critical thinking. They also help students build a useful review tool over time instead of waiting until the day before a test to start studying.

View the complete Cell Biology Warm Ups and Bell Ringers resource here.

More Biology Warm Ups and Bell Ringer Blog Posts

If you are building a larger warm up routine for your high school biology classes, these related blog posts may also be helpful:

Biology warm ups and bell ringers for the beginning of the year

Ecology warm ups and bell ringers for high school biology

Genetics warm ups and review activities for biology

DNA, RNA, and protein synthesis warm ups for biology

Evolution and classification warm ups for biology

Frequently Asked Questions

Can these cell biology warm ups be used digitally?
Yes. Digital Google Slides versions are included, so the warm ups can be used with Google Classroom, Google Drive, Microsoft OneDrive, or similar digital platforms.

Are the warm ups editable?
Yes. Editable versions are included so you can adjust the activities to fit your classroom needs.

Are these warm ups meant for first time instruction?
No. These activities are best used for review, reinforcement, practice, and discussion after students have received instruction on the topic.

Can these warm ups also be added to interactive notebooks?
Yes. Although they are primarily designed as warm ups and bell ringers, the printable pages can also be added to a student notebook or interactive notebook.

These cell biology warm ups give students a consistent routine while helping them review some of the most important topics in high school biology. They are simple to use, flexible, and helpful for keeping students engaged from the first few minutes of class.

Transpiration Lab for High School Biology: Simple Plant Transpiration Experiment


Plant transpiration can be a challenging concept for high school biology students, especially when it is only taught through notes or diagrams. This simple plant transpiration lab helps students understand what plant transpiration is by observing water loss in leaves through an easy, hands-on classroom experiment. Designed for high school biology courses, this transpiration lab makes plant water movement visible, measurable, and meaningful.

I often find that my students are woefully lacking in their knowledge of plants. When time runs short at the end of the school year, plant units are usually the first to be cut. Because I enjoy teaching plants, I make it a priority to leave time each year for a plant unit.

This version of a plant transpiration experiment is incredibly easy to set up, clean up, and take down. It uses a simple materials list and includes all the components I want in a lab activity, including data collection, graphing, critical thinking, problem solving, data analysis, and drawing conclusions. Plus, it is fun, and students always enjoy doing it.

TL;DR and want to see the activity in my TpT store? Click here.

What is Plant Transpiration?

Plant transpiration is the loss of water vapor from a plant through the leaves. As the stomata open to allow carbon dioxide to enter the leaf for photosynthesis, water vapor escapes the leaf and enters the atmosphere.

For trees and other tall plants, moving water from the roots to the top of the plant is a challenge. The major force in water transport is provided by the evaporation of water from the leaves during transpiration. As water is lost, the movement of water out of the leaf pulls water upward through the xylem all the way down to the roots. This process is known as transpiration pull.

Traditional Plant Transpiration Lab Using a Potometer

Traditional potometer setup showing plant stem and tubing used to measure water loss during a transpiration lab.

Traditionally, biology teachers used potometers to measure water loss from leaves during transpiration. Does this image bring back memories of a failed lab? Potometers are notoriously difficult to set up because it is hard to create a tight seal around the plant stem and rubber tubing. Without a good seal, results are unreliable, and students often crush the delicate stem during setup.


Simple plant transpiration experiment setup with bedding plants in beakers sealed with plastic bags to measure leaf water loss

Transpiration Lab for AP Biology and High School Biology

This simple plant transpiration lab is easy to set up, clean up, and take down, making it ideal for high school biology classrooms. Go to your local nursery or Walmart and buy some small bedding plants. I like to use begonia plants. The begonia is a good choice because it has a thick and fleshy leaf. 


Place the plants in small (250 mL) beakers. Water them thoroughly. In this experiment, you want to measure the amount of water lost from the leaves, so you have to ensure that no evaporation occurs from the soil in the beaker. Place a plastic sandwich bag around the beaker and wrap it very tightly around the stem of the plant. A bit of tape may be needed to keep the sandwich bag wrapped tightly around the stem. Only the leaf should be sticking out of the plastic bag.

The only way water is getting out of this beaker is through the leaves of this plant!

Determine the amount of water lost from the leaves by massing the entire setup. Be sure to get an initial mass before the experiment begins. In the photo shown, the initial mass was 195.57 grams.

Testable Variables in a Plant Transpiration Experiment


Now for the testable variables. What factors will cause an increase or decrease in the rate of transpiration? This lab will test 3 different environmental factors.
  • One plant will be placed in continuous light for 24 hours. 
  • One plant will be placed in front of a blowing fan for 24 hours.
  • One plant will be placed inside a plastic bag that has been spritzed with water to create high humidity.
  • The fourth plant will serve as a control.
Simple plant transpiration experiment setup showing experimental factors being tested: strong light, wind, and humidity.

Every experiment needs a control. In this transpiration lab, the control plant is not exposed to any experimental environmental factors. While the control plant is still transpiring, it does so under normal conditions, allowing students to compare results accurately.

Allow the plants to sit for 24 hours. After this time period, each plant is massed again. Students are often amazed at how much water has been lost through the leaves. All final masses should be compared to the control to determine whether the environmental factor tested caused an increase or decrease in the rate of transpiration.

There are also some uncontrolled variables in this experiment. The plants are not identical, and each has a different total leaf surface area, which can affect transpiration rates. Even so, students collect clear data showing the effects of environmental conditions on plant transpiration.

Adapting the Transpiration Lab for Different Grade Levels


Elementary: At the elementary level, it may be enough to demonstrate that water is absorbed by roots and released through leaves. Students can make predictions, form hypotheses, and practice steps of the scientific method.

Middle Grades: Middle school students can calculate the rate of water loss per minute, test additional variables such as darkness or temperature, or compare transpiration rates among different plant species.

High School Biology and AP Biology: 
High school students can remove leaves to calculate surface area and determine water loss per unit area. Students may also research mechanisms of transpiration such as cohesion, adhesion, capillary action, and transpiration pull.

After the experiment, I take the plants home and place them in my flower beds.

For additional plant kingdom resources, consider short video segments from The Private Life of Plants by David Attenborough. Many clips are available on YouTube and are typically three to five minutes long. Students always enjoy them.

Frequently Asked Questions About Plant Transpiration Labs

What is plant transpiration?
Plant transpiration is the process by which water moves from a plant’s roots, through the stem, and out of the leaves as water vapor. This water loss helps regulate temperature and supports the movement of water and nutrients throughout the plant.

What is a simple plant transpiration experiment for high school biology?
A simple plant transpiration experiment uses a live plant placed in a sealed container so that water loss occurs only through the leaves. By measuring mass before and after the experiment, students can observe and quantify transpiration.

How does this transpiration lab work for AP Biology?
This transpiration lab works well for AP Biology by allowing students to analyze variables such as light, humidity, air movement, and leaf surface area. Students can collect quantitative data, graph results, and connect observations to concepts like transpiration pull and water potential.

Why is a control important in a transpiration lab?
A control plant provides a baseline for comparison. By comparing experimental plants to a control, students can determine whether specific environmental factors increase or decrease the rate of transpiration.










Paper Chromatography Explained: Diagram, Lab Ideas, and Classroom Tips

paper chromatography experiment diagram showing pigment separation before and after solvent movementLooking for a low-prep, high-impact paper chromatography experiment to spark curiosity in your middle or high school science classroom? Paper chromatography is a classic lab technique that allows students to separate and analyze mixtures through hands-on investigation. In this paper chromatography experiment, students observe how different substances move along chromatography paper at different rates, and a simple diagram helps illustrate how solubility, polarity, and molecular properties affect separation. Whether you are introducing the scientific method, studying photosynthesis, or exploring real-world applications of chemistry and biology, paper chromatography is a versatile and visually rewarding lab to include in your curriculum.

What Is Paper Chromatography?

Paper chromatography is a simple chromatography technique used to separate the components of a mixture. This method works by taking advantage of differences in solubility and molecular interactions, allowing substances to be identified based on how far they travel up a strip of paper in the presence of a solvent. In the real world, chromatography is an essential analytical tool used in fields such as forensics, toxicology, and pharmacology. Police labs use chromatography to detect drugs and toxins in blood and urine samples, making this paper chromatography lab a meaningful way to connect classroom science to real-world STEM careers.

If you are looking for a ready-to-use paper chromatography lab for your classroom, you can find the complete student instructions, data tables, and teacher notes in my Paper Chromatography Lab resource on Teachers Pay Teachers.

Paper Chromatography Diagrams

These paper chromatography diagrams show how a solvent moves up the chromatography paper, carrying dissolved substances with it. Different components of the mixture travel at different rates based on their solubility and attraction to the paper, resulting in visible separation. This visual helps students better understand how paper chromatography works during the experiment. 

paper chromatography diagram showing separation of pigments during a chromatography experiment

Paper Chromatography Experiment Overview

👉 The process is straightforward but packed with learning potential. A small dot or line of a sample (like ink or plant pigment) is placed near the bottom of a strip of chromatography paper. This strip is then placed vertically into a container with a shallow layer of solvent. As the solvent travels up the paper by capillary action, it carries the components of the mixture with it.

Here’s why the mixture separates:

  • Solubility: More soluble substances dissolve better in the solvent and move farther up the paper.
  • Molecular Weight: Smaller molecules tend to move more quickly than larger ones.
  • Polarity: The cellulose in the paper is polar. More polar substances stick to the paper and don’t travel as far, while non-polar substances move more freely.

Chromatography Lab Ideas for the Biology or Physical Science Classroom


💧 Ink Separation

A fun and accessible introduction to chromatography is separating black ink from markers. Many black inks are actually mixtures of several colors. When placed in water, water-soluble inks spread apart into bands of dye. Not all markers will work the same way. Permanent inks may require rubbing alcohol or another solvent to separate. This gives students the opportunity to test variables and compare results across different solvents and ink types.


Paper chromatography show separation of dyes in soluble ink pens


🍃 Plant Pigment Separation

This is a favorite in high school biology, especially during units on photosynthesis or plant biology. Students can extract pigments from leaves and discover that the color they see is actually a mix of chlorophyll-a, chlorophyll-b, xanthophyll, and carotene.

Fall is a perfect time to collect a variety of leaves from trees around your school. But if you’re teaching in winter, spinach leaves from the grocery store work just as well.


Paper chromatography showing the separation of leaf pigments and calculating Rf values

Materials for a Paper Chromatography Experiment

You don’t need a fancy setup to get started. Here's a basic chromatography kit:

  • Chromatography paper (filter paper or coffee filters can work in a pinch)
  • Small containers (test tubes, jars, or beakers)
  • Solvents (water, rubbing alcohol, acetone, or other safe household solvents)
  • Samples to test (leaves, markers, food dyes, etc.)
  • Pencils, rulers, and scissors

Tip for teachers: Always test your materials and solvents in advance. Be sure to follow proper lab safety protocols, especially if using alcohols or other flammable substances.


Paper Chromatography Lab for High School Biology and Chemistry

Paper chromatography is one of the most effective chromatography labs for high school biology and chemistry classes because it allows students to visualize molecular differences directly. This chromatography experiment helps students understand key concepts such as polarity, solubility, intermolecular forces, and molecular structure.

In high school biology, paper chromatography is commonly used to separate plant pigments during photosynthesis labs. In chemistry classes, students use chromatography to analyze ink, food dyes, and other mixtures. Because the setup is simple and inexpensive, this chromatography lab is ideal for introducing experimental design, controlled variables, and data analysis.

This paper chromatography lab also helps students connect abstract molecular concepts to real, visible results, making it one of the most memorable chromatography activities in the science classroom.

how to set up Paper chromatography experiments













What Solvent Should You Use?

The answer depends on the solubility of your sample. Water works well for many inks and food dyes. For permanent markers or plant pigments, isopropyl alcohol or acetone may be more effective.

Pro Tip: Encourage students to design their own investigations. They can test multiple solvents on the same type of ink or compare different brands of markers to determine which separates most clearly.


Why Use Paper Chromatography in the Biology Lab?

Paper chromatography gives students hands-on experience with:

  • Designing and conducting controlled experiments
  • Observing molecular behavior and physical properties
  • Making qualitative and quantitative observations
  • Connecting lab techniques to real-world applications

It’s also a visually exciting lab. Watching vivid colors separate into bands on a strip of paper never gets old, and makes for excellent lab notebook sketches!

Check out this post on my blog, Leaf Pigment Chromatography, for additional photos and information. 

You may also be interested in my osmosis lab using dialysis tubing and my potato osmosis experiment, which help students understand diffusion, osmosis, and molecular transport in cells. Check out these blog posts:


Frequently Asked Questions About Paper Chromatography

What is chromatography used for?
Chromatography is used to separate and analyze mixtures by observing how different substances move through a medium. Scientists use chromatography in fields such as forensics, medicine, environmental science, and food testing.

Why does paper chromatography work?
Paper chromatography works because different substances have different attractions to the paper and the solvent. These differences cause components of a mixture to travel at different speeds, resulting in separation.

What can students learn from a paper chromatography experiment?
Students learn key lab skills such as experimental design, data collection, observation, and analysis while exploring concepts like solubility, polarity, and molecular interactions.

How do you perform a paper chromatography lab in the classroom?

To perform a paper chromatography lab, students place a small sample of ink or plant pigment near the bottom of chromatography paper and suspend the paper in a solvent. As the solvent travels upward, it separates the mixture into its components based on solubility and polarity. Students can measure how far each component travels and calculate Rf values to analyze the results and compare substances.

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Ready to Try This With Your Students?

These chromatography labs have been classroom tested and include complete student instructions, answer keys, and teacher guidance for easy implementation. If you're looking for classroom-ready chromatography labs, I have two student-tested resources that include everything you need: detailed procedures, printable lab handouts, answer keys, and grading rubrics.

🔬 Matter Lab Stations: Ink Separation – Perfect for introducing lab skills and the scientific method in both middle and high school.

🌿 Plant Pigment Chromatography Lab – Ideal for exploring photosynthesis and leaf pigments in your high school biology class.

Both labs are designed to save you prep time while providing meaningful, standards-based instruction. You can find them in my Teachers Pay Teachers store.