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Real Science Teaching. Real Classroom Experience.

I’m Amy Brown, a veteran high school biology and chemistry teacher, wife, and mom who understands the daily reality of lesson planning, grading, meetings, and everything in between. I know what it feels like to have too much to do and not enough time to do it.

After decades in the classroom, I’ve created rigorous, classroom-tested biology and chemistry resources that save you planning time while still delivering strong, meaningful science instruction. Every lab, activity, and lesson is designed to move students beyond memorization and into real scientific thinking.

If you want your students excited about science and thinking deeply without spending your entire weekend planning, you’re in the right place.

Amy Brown Biology and Chemistry Teacher

“I just love getting kids hooked on science.”

The Amazing Story of the Lord Howe Island Stick Insect























I just read this article on NPR.com.  The article is about the stick insects found on Lord Howe's Island.  The insects were thought to be extinct.  But a small colony was found elsewhere.  (I don't want to give away too much!  Please click the link below to read the full article.)  This is just an amazing story.  I love nature and biodiversity. This is one of the best articles I have read in a long, long time.  And the pictures are amazing!!


Amazing, right?


Catalase Enzyme Lab: Liver and Potato Experiment for High School Biology


catalase enzyme lab with liver and potato showing oxygen bubbles from hydrogen peroxide reaction free biology lab activity


This catalase enzyme lab uses liver and potato tissues to demonstrate enzyme activity in a clear and engaging way for high school biology students. In this catalase lab experiment, students observe how catalase breaks down hydrogen peroxide into water and oxygen, producing visible bubbles that show enzyme activity in real time. This enzyme lab is an excellent hands-on activity for teaching enzyme function, enzyme specificity, and biological reactions.

This catalase enzyme lab is an excellent high school biology experiment for teaching enzyme activity, biological catalysts, and biochemical reactions using simple classroom materials. This lab is one of the most popular enzyme experiments for teaching enzyme activity and is easy to set up using common classroom materials.

You can download this free catalase enzyme lab activity here to use with your biology students.

What concepts are covered and reinforced?

1.  Enzymes speed up the rate of biological chemical reactions.
2.  The same enzymes are found in both plant and animal cells.
3.  Enzymes are highly specific for the reaction they catalyze.
4.  Enzymes carry out their functions more efficiently than catalysts.
5.  Enzymes can be denatured by excessive heat.

Why Catalase Is Found in Both Plant and Animal Cells

Catalase is an enzyme that is commonly found in both plant and animal tissues. Its function is to break down hydrogen peroxide, a toxic byproduct of cellular respiration and other metabolic reactions, into water and oxygen. Because hydrogen peroxide is continuously produced inside living cells, catalase plays an essential role in protecting plant and animal cells by preventing harmful buildup of this toxic substance.


Manganese dioxide is a non-biological catalyst. Like enzymes, catalysts speed up chemical reactions, but unlike enzymes, catalysts are not specific to a single reaction. This comparison helps students understand the unique properties of enzymes and how enzyme activity differs from inorganic catalysts.  

This catalase enzyme lab uses common materials to demonstrate enzyme activity in plant and animal tissues.

Materials for the Catalase Enzyme Lab Using Liver and Potato


  • Fresh plant and animal tissue (Potato and Liver)
  • Boiled animal tissue (Liver)
  • 3% hydrogen peroxide
  • Manganese dioxide
  • Test tubes and test tube rack
  • Sand
  • Mortar and pestle

Catalase is found in all eukaryotic cells, which is why this enzyme lab works using many types of fresh, living tissues. Liver is an excellent source of catalase from animal cells, and potato tissue provides a reliable source of catalase from plant cells. Comparing catalase activity in liver and potato tissues allows students to observe enzyme activity in both plant and animal cells. This comparison helps students understand that catalase is present in many different living tissues and plays an essential role in protecting cells.


What Happens When Catalase Breaks Down Hydrogen Peroxide?

Students will observe bubbles forming as catalase breaks down hydrogen peroxide into water and oxygen gas. These bubbles are oxygen released during the catalase enzyme reaction. Fresh liver and potato tissues produce a strong bubbling reaction because they contain active catalase, while boiled tissues produce little or no reaction because heat has denatured the enzyme.


Students absolutely love this catalase enzyme lab because they can immediately observe enzyme activity as bubbles of oxygen form during the reaction. 

This free catalase enzyme lab activity includes complete student instructions and a teacher guide to help you easily implement this engaging enzyme experiment in your classroom.


Frequently Asked Questions About the Catalase Enzyme Lab

Why do both plant and animal cells contain catalase?

Catalase is found in both plant and animal cells because all living cells produce hydrogen peroxide during normal metabolic processes. Catalase protects cells by breaking hydrogen peroxide down into water and oxygen.

Why is liver often used in catalase labs?

Liver cells contain large amounts of catalase because the liver plays a major role in detoxification. This makes liver an excellent tissue for demonstrating catalase enzyme activity in lab experiments.

Why do boiled tissues show little or no catalase activity?
Boiling denatures the catalase enzyme, changing its structure so it can no longer function properly. This prevents catalase from breaking down hydrogen peroxide.

What causes the bubbles in a catalase enzyme lab?
The bubbles are oxygen gas released when catalase breaks down hydrogen peroxide into water and oxygen. This visible reaction demonstrates enzyme activity.

Related Blog Posts About Enzymes and Enzyme Labs:

Everything About Enzymes!

The Affect of the Enzyme Amylase on Starch

Why Do Living Cells Need Buffers? A Homeostasis Lab for Biology

Another engaging activity to include in your biochemistry unit is this food testing lab for organic compounds, where students identify sugars, starches, proteins, and lipids using indicator tests.

Related Enzyme Labs and Biology Activities

These enzyme labs and biology activities help students further explore enzyme function, enzyme activity, and biochemical reactions in living cells.

Enzyme Lab: The Effect of pH on Living Cells

Another FREEBIE! Cellular Respiration Word Game Review



Great review with a secret message!

This is a FREE download!  Click red text below to receive your free copy of this worksheet:

Currently, our Biology I classes are covering cellular respiration. This may seem odd to many of you.  Who waits until the end of February to teach cellular respiration?  The answer to this question:  Those of us who teach on a trimester system! Teaching on a trimester system is the topic of a whole different blog article!! We are currently teaching cell respiration to students who began their biology course at the beginning of the second trimester.

Cellular respiration is one of the hardest topics a biology teacher has to teach during their course.  Its very abstract nature makes it particularly hard for many students to comprehend.  In my experience, the best approach is to provide as many opportunities as possible for the students to review and work with the information.  To give my students some extra review on the vocabulary words used in this unit,  I wrote this short review worksheet.

The printable lesson is perfect for traditional classroom settings, and the paperless, digital Google Apps version is perfect for distance learning and 1:1 classrooms. 

On the left side of the worksheet, a definition is given.  The student must write the correct term in the spaces to the right.  There are 28 vocabulary words used on this review worksheet.  The vocab words are:  pyruvic acid, protons, glucose, aerobic, matrix, adenosine triphosphate, ATP synthase, water, adenine, respiration, oxygen, lactic acid, Kreb's cycle, alcoholic, phosphate, glycolysis, citric acid, NADH, electron transport chain, cristae, mitochondria, carbon dioxide, fermentation, cytoplasm, thirty eight, anaerobic, ribose, and acetate.


The boxed in letters will spell out a secret message.  The students record these letters in the blanks at the end of the worksheet to reveal the hidden message.   WARNING:  Make sure you read the hidden message before you pass out this worksheet.  It involves the giving of extra credit points on the test!  This is such a hard concept for so many students that I am happy to offer them some extra credit points if they complete this review worksheet.

This is just one of several types of review I do for this topic.  I am happy to share this vocabulary worksheet with you.  I hope you find it useful.


Happy Teaching!!

Density Lab: Identifying Unknown Metals in High School Chemistry

Density of metals chemistry lab for grades 9–12 in which students identify unknown metal samples using density.

Click the image to view Density Lab: Identifying Unknown Metals on TpT.

A density lab for high school chemistry or physical science gives students a hands-on way to connect mass, volume, and density. In this investigation, students use a balance and water displacement to determine the density of four unknown metals, then use a reference table to identify each sample. The lab also reinforces percent error calculations, graphing, and data analysis.

I like this lab because it brings several important science skills together in one manageable investigation. Students have a reason to measure carefully, their calculations help them solve a problem, and the follow-up questions ask them to think about what their numbers mean. It works well after students have learned how to calculate density.

Using Density to Identify Unknown Metals

Density is the ratio of mass to volume. Students may be comfortable substituting numbers into the equation, but working with unknown samples helps them understand why density is useful. A larger piece of a metal has more mass and occupies more volume than a smaller piece of the same metal, yet both have the same density under the same conditions.

Density = Mass ÷ Volume

For this lab, students receive four metal samples labeled A, B, C, and D. They measure the mass of each sample with a balance and determine its volume using water displacement. After calculating density, they compare their results with the values in the supplied reference table and choose the closest reasonable match.

This is also a useful opportunity to discuss density as a physical property. Students identify the samples without changing their chemical composition. The analysis questions ask them to consider other observable properties and whether density alone is always enough to identify an unknown substance. If your students need more practice with physical and chemical properties, my post about matter and change task cards offers another way to review those concepts.

Measuring Volume with Water Displacement

Before students begin, review how to read the bottom of the meniscus at eye level and how to record measurements from the graduated cylinder. Students record the initial water volume, carefully lower a metal sample into the water, and record the final volume. The difference between those readings is the volume of the metal.

Volume of Metal = Final Water Volume − Initial Water Volume

Remind students to slide the sample gently into the cylinder, fully submerge it, and check for trapped air bubbles. A small error in the volume measurement can make a noticeable difference in the calculated density, especially when the sample displaces only a small amount of water. That gives you something concrete to discuss when students compare their experimental values with the reference table.

The lab includes percent error calculations, so students can evaluate how closely their measurements agree with the accepted densities. Use their results to discuss measurement precision and possible sources of error. For more ideas about preparing students to work successfully with lab equipment, see Taking Students to the Laboratory.

Graphing Reinforcement Is an Important Part of This Lab

One of my favorite parts of this density lab is the graphing reinforcement. Graphing is a skill students need to practice throughout the year, and this activity connects the graph to the density concepts they have just used in the investigation.

The graphing section provides mass and volume data for five pieces of aluminum of different sizes. Students plot volume on the x-axis and mass on the y-axis. They must choose an appropriate scale, label the axes with units, give the graph a title, and plot the data carefully. The resulting pattern helps them see that mass and volume are directly proportional for a material with a fixed density.

Students then use their graph to determine the mass of an aluminum sample with a given volume and the volume of a sample with a given mass. One question asks them to predict a mass beyond the range of the supplied data, giving you an opportunity to discuss extrapolation. These questions require students to use the graph as a source of information.

If graphing is a skill you are working to strengthen in your classes, you may also enjoy Science Graphing Activities: Why Students Need to Learn Graphing by Hand. The density lab provides another opportunity to revisit those skills within your chemistry content.

Four-page editable density lab student handout with data tables, analysis questions, calculation space, and a graphing grid.

The four-page handout includes space for calculations, written answers, and the graphing activity.

Choosing Metal Samples and Preparing the Lab

You can choose the metals that work best for your classroom and available supplies. I most often use copper, aluminum, lead, and zinc. Choose samples that fit safely inside your graduated cylinders and have densities represented in the reference table. Label them with letters so students use their measurements to determine their identities.

 The teacher guide includes ordering information for metal strips, along with preparation instructions and suggestions for setting up the investigation.

An Optional Comparison: Measuring Metal Strips Directly

If you use rectangular metal strips, their volume can also be calculated from their dimensions. Students measure the length and width with a metric ruler and use the strip’s thickness as the third dimension. Because thin strips can be difficult to measure accurately with a ruler, use the thickness listed on the package.

Volume = Length × Width × Thickness

Be sure students convert all three measurements to the same unit before multiplying. Measurements in centimeters give a volume in cubic centimeters, and 1 cm3 is equivalent to 1 mL. Comparing this result with the water displacement measurement can lead to a useful discussion about the strengths and limitations of each method.

Two Editable Student Handout Formats

The lab includes two versions of the student handout with the same investigation and questions. The four-page version provides space for students to record data, show calculations, write answers, and construct their graph. The two-page paper-saving version allows students to complete their work on their own notebook paper and graph paper, so you can reuse the directions with another class.

Both versions are editable, allowing you to adjust the directions or questions for your students. The teacher guide includes preparation and setup instructions, materials ordering information, tips, sample data, and detailed responses to the questions.

A Density Lab for Your Chemistry Math Unit

This lab is included in my Chemistry Math Unit: Scientific Measurements, Calculations, and Graphing. It gives students an opportunity to apply measurement, calculations, and graphing in a hands-on investigation. If you already own that unit, you already have this lab.

For ideas about building those skills in your chemistry classes, visit my post on chemistry measurement and math skills. You can also explore The Ultimate Guide to Teaching Science Process Skills for ways to reinforce measurement, data analysis, and scientific thinking throughout the year.

If you only need the individual investigation, Density Lab: Identifying Unknown Metals is available separately on TpT. Click the link or any image in this post to view the resource and its preview.

Citizen Science.....Revisited....Get Involved!



Back in December, I wrote a blog article on Citizen Science from the Scientific American website.  (Click here to read the original blog post.)  I like this idea so much that I wanted to follow up on it.

This week end you can participate in The Great Backyard Bird Count.  This event will be taking place from Friday, Feb 17 through Monday, Feb 20.    From their web site:  "The Great Backyard Bird Count is a 4 day event that engages bird watchers of all ages in counting birds to create a real-time snapshot of where the birds are across the continent.  Anyone can participate, from beginning bird watchers to experts.  It takes as little as 15 minutes on one day, or you can count for as long as you like each day of the event.  It's free, fun and easy - and it helps the birds".

It is not too late to sign up.  You can find all of the information here.

What is the count used for?  The data is used by scientists to answer questions about populations, migration patterns, the effect of environmental influences on populations, how weather patterns affect populations, and much more.  The details can be seen by clicking the link above.

We do not have school on Monday since it is President's Day.  I am the sponsor of the science club at our school.  The 30 member science club is meeting at our local animal shelter on that day for a day of community service.  We will be raking, cleaning up the outdoor play areas for the dogs, scooping poop along the walking trail, and no doubt cleaning out animal cages of all types.  But we will also be participating in the Great  Backyard Bird Count.  Our animal shelter is on the edge of town in a wooded area.  I just hope we will be able to identify and count the numerous birds that we will see!!

This is a fabulous activity for the entire family and it is not too late to sign up and join in on the fun!  Plan an outdoor event with your family this week end and include the Great Backyard Bird Count as part of your activities.

 Finally, I want to share this new link with you.  This is an article I just read on Nature.com.  It is called, "Citizen Science Goes Extreme."  This was a well written and very informative article about the need for citizens world-wide to participate in scientific research and the preservation of habitats.

Valentine’s Day Biology Activities for High School

If you are looking for Valentine’s Day biology activities that are actually meaningful for high school students, you are not alone. Finding engaging, age appropriate science activities that still connect to your curriculum can feel like a challenge. The good news is that you can bring a little seasonal fun into your classroom without sacrificing rigor.

Here are a few of my favorite Valentine’s Day science activities that work well in high school biology and chemistry classes.

Daphnia Heart Rate Lab for Valentine’s Day

For me, it just so happens that I usually manage to be teaching the circulatory system around Valentine’s Day. Although not nearly as fun as exchanging valentines and getting treat bags, at least it does involve the heart.

One of my favorite labs is Measuring the Heart Rate in Daphnia. It is a two part activity. In the first part, students explore the effect of temperature on heart rate. In the second part, students design their own experiment to test a different variable and see how it affects heart rate. It is a really fun lab, and students love it.

If you want the full lab setup, procedure, and more teaching details, you can read more here: Measuring Heart Rate in Daphnia

Quick Valentine’s Day Science Activity

Granted, the Daphnia lab takes some planning and probably takes two class periods to complete. If you are looking for something quick and very easy, take a look at my Science of Valentine’s Day video from my YouTube channel, the Amy Brown Science Learning Lab. The four minute video is free for your classroom use.

I also wrote a fun acrostic puzzle activity to accompany the video. Students answer questions to decipher three really corny Valentine jokes. You can view the puzzles here: The Science of Valentine’s Day

Chemistry Valentine Activity

Finally, for chemistry teachers, I have a Valentine’s Day Color by Number activity with a Valentine theme. Students practice writing chemical formulas and naming compounds while uncovering the hidden picture.

This one works especially well as a quick review activity, an easy day lesson, or a simple option during a busy week. You can view it here: Chemistry Valentine’s Day Color by Number

Connecting to Other Biology Topics

This Daphnia lab also fits well into other units beyond the circulatory system. You can use it when discussing ecology, experimental design, or data analysis.

If you are teaching ecology, you might also like to read about this activity: Population Ecology Lab

Frequently Asked Questions

What are good Valentine’s Day biology activities for high school?
Labs like the Daphnia heart rate experiment, short science videos with guided questions, and review activities all work well while keeping the focus on content.

Can you do labs on Valentine’s Day?
Yes. As long as the activity connects to your standards, it can be a great way to keep students engaged during a high energy day.

What is a quick Valentine’s Day science activity?
Short videos, bell ringers, or simple review activities like puzzles or color by number are easy to implement with very little prep.

Daphnia Heart Rate Lab for Biology that Students Love

Watch Their Hearts Beat: A Daphnia Lab That Brings Biology to Life

If you’re looking for a biology lab that’s easy to set up, actually engages students, and still hits real science skills, this Daphnia heart rate lab is one of my favorites. Students observe the beating heart of a live Daphnia under the microscope, test the effect of temperature on heart rate, and then extend the investigation by designing their own experiment. It is the kind of lab that feels exciting for students while still building serious biology skills.

Daphnia are tiny aquatic crustaceans, sometimes called water fleas. Their transparent exoskeleton makes the beating heart visible under low power, which makes them ideal for a lab on physiology, environmental effects on organisms, graphing, and experimental design. This lab gives students the rare chance to work with a living organism and collect real data in a way that is visual, concrete, and highly engaging.

This lab is also classroom friendly. It is low prep, easy to implement, and flexible enough to use with standard biology, honors biology, and AP Biology students. If you want a ready to use resource with student handouts, graphing pages, extension activities, and teacher guidance, you can find it here: Measuring Heart Rate in Daphnia Lab.

What Students Observe During This Daphnia Heart Rate Lab

In this lab, students observe the beating heart of Daphnia and investigate how temperature affects heart rate. Because Daphnia are ectotherms, their body temperature changes with the surrounding environment, which makes temperature an excellent variable for students to study. As students compare conditions, they can see physiology in action instead of only reading about it in a textbook.

This first part of the lab gives students a structured, guided investigation. They collect data, graph their results, and answer analysis questions. The experience helps students connect temperature, metabolism, and organismal function while practicing core science skills in a meaningful context.

Helping Students Locate the Daphnia Heart

One of the biggest challenges in a Daphnia heart rate lab is helping students find the heart. The digestive system of Daphnia is active, and students often mistake that movement for the heartbeat. The heart is located just behind the head on the dorsal surface of the body, and it appears as a very small, clear, transparent beating sac.

That is one reason this resource includes a diagram for each lab station. Having a visual reference makes a big difference and helps students begin the lab with more confidence. It also reduces frustration and saves class time because students are more likely to focus on the correct structure from the start.

If you teach body systems, you might also like this related post on the circulatory system interactive notebook.

How This Lab Works in Your Classroom

This resource actually includes three different activities, which makes it much more than a simple one day microscope lab.

First, students follow the lab procedure to determine how temperature affects the heart rate of Daphnia. This is the structured part of the lab and works well for a wide range of learners.

Second, students can design their own experiment to test the effect of a new variable on Daphnia heart rate. This is where the lab becomes especially valuable because students move beyond following directions and begin thinking like scientists.

Third, honors and AP Biology students can complete the Q10 Temperature Coefficient worksheet for a deeper quantitative extension.

This structure gives you flexibility. You can stop after the guided temperature lab, or you can extend the activity into a more advanced investigation. That makes it easy to adapt for your schedule, your students, and the level of rigor you want.

Extending the Lab with Student Designed Experiments

One of the strongest parts of this Daphnia lab is the student designed experiment. After students complete the guided temperature investigation, they can use the scientific method to design an experiment that tests the effect of a different variable on heart rate. In my classroom, this is where the fun really begins.

Students state a hypothesis, identify control and experimental groups, carry out the procedure, collect data, graph their results, and write a conclusion based on the evidence. This part of the lab takes more time, but it also leads to some of the best learning. Students quickly discover that real experimentation requires careful planning, revision, and problem solving.

If you are nervous about student designed experiments, do not be. With guidance on the front end, students often surprise you with their creativity and persistence. This part of the lab is excellent for reinforcing experimental design in a way that feels authentic rather than forced.

Differentiation for Honors and AP Biology

This lab also includes a Q10 Temperature Coefficient worksheet, which makes it especially useful for honors and AP Biology classes. Students can move beyond simple observation and graphing into a more advanced analysis of how temperature affects biological processes.

That means this resource is not limited to one level of biology. The first activity is accessible enough for many high school students, while the later extensions provide the rigor needed for advanced learners.

What This Looks Like in Your Classroom

Students assemble a simple habitat chamber using depression slides, a pipet, and other basic materials. They observe the beating heart under the microscope, compare the effect of different temperatures, record heart rate data, and graph their results. From there, you can extend into a student designed investigation and formal lab report if you choose.

This is the kind of lab that keeps students interested because they are working with a living organism and collecting real data. It also gives you a natural opportunity to teach careful observation, ethical treatment of organisms, graphing, analysis, and experimental design all within one lab sequence.

Please note: Teach students to respect all living organisms. No harm should come to the Daphnia during this experiment.

Why Teachers Love This Lab

This lab is a favorite of mine every year because it combines genuine student excitement with meaningful biology content. Students love looking at living organisms under the microscope, and Daphnia are especially fascinating because the beating heart is visible. At the same time, the lab goes far beyond novelty.

This investigation helps students practice:

  • Graphing and analyzing data
  • Comparing variables and controls
  • Designing and carrying out investigations
  • Problem solving and critical thinking
  • Connecting environmental change to organismal response

It also supports the kind of hands on biology experience that teachers want in the classroom. Working with living organisms helps students build curiosity, observation skills, and appreciation for the living world.

When the lab is over, my Daphnia spend the rest of their days in my Elodea tank.

What Is Included in This Resource

This Daphnia heart rate lab includes everything you need for successful implementation:

  • Editable lab handouts ready to print and use
  • Complete instructions
  • Diagram for each lab station to help students locate the beating heart
  • Student designed experiment handouts
  • Q10 Temperature Coefficient worksheet
  • A teacher guide with setup instructions, tips, tricks, and suggestions
  • A complete answer key with sample data, sample graph, and answers

That combination makes this much more than a simple worksheet or one day activity. It is a complete lab sequence that can be adapted to the needs of your classroom.

Ready to Try This Daphnia Heart Rate Lab?

If you are looking for a complete, ready to use Daphnia heart rate lab that saves prep time and gives students a meaningful biology experience, this resource is a great fit. It combines microscope work, physiology, graphing, scientific method, and optional advanced extensions in one engaging package.

Click here to view the resource: Measuring Heart Rate in Daphnia Lab